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human prostate carcinoma cell lines pc3  (ATCC)


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    ATCC human prostate carcinoma cell lines pc3
    Human Prostate Carcinoma Cell Lines Pc3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 14585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+prostatic+carcinoma+cell+lines+pc3/PC-3/pmc12481549__se5c01433_si_001-86-1-11
    Average 99 stars, based on 14585 article reviews
    human prostate carcinoma cell lines pc3 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Methylation:

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC ® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.

    Article Title: Allele-specific methylation of the PSA promoter in prostate cells: A new translational marker for the differential diagnosis of prostate cancer
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435™) and LNCaP (ATCC CRL1740™) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) from the DSMZ, German Collection of Microorganisms and Cell Cultures, GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories.. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Methylation Sequencing:

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC ® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.

    Article Title: Allele-specific methylation of the PSA promoter in prostate cells: A new translational marker for the differential diagnosis of prostate cancer
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435™) and LNCaP (ATCC CRL1740™) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) from the DSMZ, German Collection of Microorganisms and Cell Cultures, GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories.. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Expressing:

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC ® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.

    Article Title: Allele-specific methylation of the PSA promoter in prostate cells: A new translational marker for the differential diagnosis of prostate cancer
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435™) and LNCaP (ATCC CRL1740™) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) from the DSMZ, German Collection of Microorganisms and Cell Cultures, GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories.. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Quantitative RT-PCR:

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC ® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.

    Article Title: Allele-specific methylation of the PSA promoter in prostate cells: A new translational marker for the differential diagnosis of prostate cancer
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435™) and LNCaP (ATCC CRL1740™) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) from the DSMZ, German Collection of Microorganisms and Cell Cultures, GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories.. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    RNA Sequencing:

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 ◦C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC ® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO 2 according to the manufacturer’s protocol.

    Article Title: Allele-specific methylation of the PSA promoter in prostate cells: A new translational marker for the differential diagnosis of prostate cancer
    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435™) and LNCaP (ATCC CRL1740™) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) from the DSMZ, German Collection of Microorganisms and Cell Cultures, GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories.. All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.All cell lines were cultured at 37 °C in a humidified incubator with 5% CO2 according to the manufacturer’s protocol.



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    Figure 2. The methylated in <t>PC3</t> and unmethylated in LNCaP cells proximal PSA promoter is cancer specific. Bisulfite sequencing analyses of the PSA PP in PC3 and LNCaP cells. A and a represent the parental alleles. At the bottom of the illustrations, the methylation status of the CpGs and CCWGG motif is shown.
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    Figure 2. The methylated in <t>PC3</t> and unmethylated in LNCaP cells proximal PSA promoter is cancer specific. Bisulfite sequencing analyses of the PSA PP in PC3 and LNCaP cells. A and a represent the parental alleles. At the bottom of the illustrations, the methylation status of the CpGs and CCWGG motif is shown.
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    Image Search Results


    Figure 2. The methylated in PC3 and unmethylated in LNCaP cells proximal PSA promoter is cancer specific. Bisulfite sequencing analyses of the PSA PP in PC3 and LNCaP cells. A and a represent the parental alleles. At the bottom of the illustrations, the methylation status of the CpGs and CCWGG motif is shown.

    Journal: International journal of molecular sciences

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.

    doi: 10.3390/ijms26031243

    Figure Lengend Snippet: Figure 2. The methylated in PC3 and unmethylated in LNCaP cells proximal PSA promoter is cancer specific. Bisulfite sequencing analyses of the PSA PP in PC3 and LNCaP cells. A and a represent the parental alleles. At the bottom of the illustrations, the methylation status of the CpGs and CCWGG motif is shown.

    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).

    Techniques: Methylation, Methylation Sequencing

    Figure 4. The DNMT profile in cells with PSA monoallelic methylation is similar overall. (a) DNMT1 is downregulated in LNCaP cells with an unmethylated PSA promoter. In contrast, it is activated in PC3 cells, where the PSA promoter is biallelically methylated. The relative expression of DNMT1, DNMT3A, and DNMT3B mRNA was assessed by qRT-PCR. The results were normalized to that of TBP expression. All data are presented as the mean ± SD of three independent experiments. (b) DNMT expression levels determined by RNA sequencing in normal prostate tissues. RNA sequencing was performed on tissue samples from 95 people. Image adapted from NCBI Bioinformatics Database resources: BioProject, PRJEB4337 [13].

    Journal: International journal of molecular sciences

    Article Title: Allele-Specific CG/CCWGG Methylation of the PSA Promoter Discriminates Aggressive, Indolent, and Benign Prostate Cell Lines and Is Involved in the Regulation of PSA Expression.

    doi: 10.3390/ijms26031243

    Figure Lengend Snippet: Figure 4. The DNMT profile in cells with PSA monoallelic methylation is similar overall. (a) DNMT1 is downregulated in LNCaP cells with an unmethylated PSA promoter. In contrast, it is activated in PC3 cells, where the PSA promoter is biallelically methylated. The relative expression of DNMT1, DNMT3A, and DNMT3B mRNA was assessed by qRT-PCR. The results were normalized to that of TBP expression. All data are presented as the mean ± SD of three independent experiments. (b) DNMT expression levels determined by RNA sequencing in normal prostate tissues. RNA sequencing was performed on tissue samples from 95 people. Image adapted from NCBI Bioinformatics Database resources: BioProject, PRJEB4337 [13].

    Article Snippet: Human prostatic carcinoma cell lines PC3 (ATCC® CRL1435TM) and LNCaP (ATCC CRL-1740TM) were purchased from the American Type Culture Collection (ATCC); BPH1 (DSMZ no.: ACC 143) came from the DSMZ, German Collection of Microorganisms and Cell Cultures; and GmbH and the human prostatic epithelial cell line (HPrEpiC) were obtained from the ScienCell Research Laboratories (Walkersville, MD, USA).

    Techniques: Methylation, Expressing, Quantitative RT-PCR, RNA Sequencing

    Figure 10. Effect of compound 6 (92.16 lM) on DNA ploidy flow cytometric analysis of PC-3 cells after 24 h.

    Journal: Journal of enzyme inhibition and medicinal chemistry

    Article Title: Design, synthesis, and biological evaluation of novel ciprofloxacin derivatives as potential anticancer agents targeting topoisomerase II enzyme.

    doi: 10.1080/14756366.2022.2136172

    Figure Lengend Snippet: Figure 10. Effect of compound 6 (92.16 lM) on DNA ploidy flow cytometric analysis of PC-3 cells after 24 h.

    Article Snippet: Human bladder cancer (T-24) and human prostate carcinoma (PC3) cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA USA).

    Techniques:

    Figure 11. Effect of compound 8a (10.95 lM) on DNA-ploidy flow cytometric analysis of PC-3 cells after 24 h.

    Journal: Journal of enzyme inhibition and medicinal chemistry

    Article Title: Design, synthesis, and biological evaluation of novel ciprofloxacin derivatives as potential anticancer agents targeting topoisomerase II enzyme.

    doi: 10.1080/14756366.2022.2136172

    Figure Lengend Snippet: Figure 11. Effect of compound 8a (10.95 lM) on DNA-ploidy flow cytometric analysis of PC-3 cells after 24 h.

    Article Snippet: Human bladder cancer (T-24) and human prostate carcinoma (PC3) cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA USA).

    Techniques:

    Figure 12. Bar presentation showing effects of compounds 6 (92.16 lM) and 8a (10.95lM) on DNA-ploidy flow cytometric analysis of PC-3 cells after 24 h.

    Journal: Journal of enzyme inhibition and medicinal chemistry

    Article Title: Design, synthesis, and biological evaluation of novel ciprofloxacin derivatives as potential anticancer agents targeting topoisomerase II enzyme.

    doi: 10.1080/14756366.2022.2136172

    Figure Lengend Snippet: Figure 12. Bar presentation showing effects of compounds 6 (92.16 lM) and 8a (10.95lM) on DNA-ploidy flow cytometric analysis of PC-3 cells after 24 h.

    Article Snippet: Human bladder cancer (T-24) and human prostate carcinoma (PC3) cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA USA).

    Techniques:

    Figure 15. Representative dot plots of PC-3 cells treated with 6 (92.16 lM) for 24 h and analysed by flow cytometry after double staining of the cells with annexin-V FITC and PI.

    Journal: Journal of enzyme inhibition and medicinal chemistry

    Article Title: Design, synthesis, and biological evaluation of novel ciprofloxacin derivatives as potential anticancer agents targeting topoisomerase II enzyme.

    doi: 10.1080/14756366.2022.2136172

    Figure Lengend Snippet: Figure 15. Representative dot plots of PC-3 cells treated with 6 (92.16 lM) for 24 h and analysed by flow cytometry after double staining of the cells with annexin-V FITC and PI.

    Article Snippet: Human bladder cancer (T-24) and human prostate carcinoma (PC3) cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA USA).

    Techniques: Flow Cytometry, Double Staining

    Figure 16. Representative dot plots of PC-3 cells treated with 8a (10.95 lM) for 24 h and analysed by flow cytometry after double staining of the cells with annexin-V FITC and PI.

    Journal: Journal of enzyme inhibition and medicinal chemistry

    Article Title: Design, synthesis, and biological evaluation of novel ciprofloxacin derivatives as potential anticancer agents targeting topoisomerase II enzyme.

    doi: 10.1080/14756366.2022.2136172

    Figure Lengend Snippet: Figure 16. Representative dot plots of PC-3 cells treated with 8a (10.95 lM) for 24 h and analysed by flow cytometry after double staining of the cells with annexin-V FITC and PI.

    Article Snippet: Human bladder cancer (T-24) and human prostate carcinoma (PC3) cell lines were obtained from American Type Culture Collection (ATCC; Manassas, VA USA).

    Techniques: Flow Cytometry, Double Staining